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anti ca9 pe antibody  (Miltenyi Biotec)


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    Miltenyi Biotec anti ca9 pe antibody
    Anti Ca9 Pe Antibody, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ca9/Carbonic+Anhydrase+9+Antibody%2C+anti-human%2C+REAfinity/pm41663542-345-10-13
    Average 94 stars, based on 10 article reviews
    anti ca9 pe antibody - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Purification:

    Article Title: A neoantigen vaccine generates antitumour immunity in renal cell carcinoma
    Article Snippet: .. CA9-positive tumour cells were immunomagnetically purified (using Miltenyi anti-CA9 PE antibody and anti-PE microbeads) from freshly dissociated tumour and cultured for 48–72 h in OptiMEM GlutaMax medium (Gibco) containing 5% FBS, 1 mM sodium pyruvate, 100 U ml –1 penicillin and streptomycin, 50 μg ml –1 gentamicin, 5 μg ml –1 insulin and 5 ng ml –1 EGF. .. RNA was extracted using a Qiagen RNeasy kit, and RNA-seq of this low-input RNA was performed using a modified version of the Smart-seq2 protocol (using the NEBNext Single Cell/Low Input cDNA Synthesis and Amplification module) as previously described .

    Article Title: A neoantigen vaccine generates antitumour immunity in renal cell carcinoma.
    Article Snippet: .. CA9-positive tumour cells were immunomagnetically purified (using Miltenyi anti-CA9 PE antibody and anti-PE microbeads) from freshly dissociated tumour and cultured for 48–72 h in OptiMEM GlutaMax medium (Gibco) containing 5% FBS, 1 mM sodium pyruvate, 100 U ml–1 penicillin and streptomycin, 50 μg ml–1 gentamicin, 5 μg ml–1 insulin and 5 ng ml–1 EGF. .. RNA was extracted using a Qiagen RNeasy kit, and RNA-seq of this low-input RNA was performed using a modified version of the Smart-seq2 protocol (using the NEBNext Single Cell/Low Input cDNA Synthesis and Amplification module) as previously described3.

    Cell Culture:

    Article Title: A neoantigen vaccine generates antitumour immunity in renal cell carcinoma
    Article Snippet: .. CA9-positive tumour cells were immunomagnetically purified (using Miltenyi anti-CA9 PE antibody and anti-PE microbeads) from freshly dissociated tumour and cultured for 48–72 h in OptiMEM GlutaMax medium (Gibco) containing 5% FBS, 1 mM sodium pyruvate, 100 U ml –1 penicillin and streptomycin, 50 μg ml –1 gentamicin, 5 μg ml –1 insulin and 5 ng ml –1 EGF. .. RNA was extracted using a Qiagen RNeasy kit, and RNA-seq of this low-input RNA was performed using a modified version of the Smart-seq2 protocol (using the NEBNext Single Cell/Low Input cDNA Synthesis and Amplification module) as previously described .

    Article Title: A neoantigen vaccine generates antitumour immunity in renal cell carcinoma.
    Article Snippet: .. CA9-positive tumour cells were immunomagnetically purified (using Miltenyi anti-CA9 PE antibody and anti-PE microbeads) from freshly dissociated tumour and cultured for 48–72 h in OptiMEM GlutaMax medium (Gibco) containing 5% FBS, 1 mM sodium pyruvate, 100 U ml–1 penicillin and streptomycin, 50 μg ml–1 gentamicin, 5 μg ml–1 insulin and 5 ng ml–1 EGF. .. RNA was extracted using a Qiagen RNeasy kit, and RNA-seq of this low-input RNA was performed using a modified version of the Smart-seq2 protocol (using the NEBNext Single Cell/Low Input cDNA Synthesis and Amplification module) as previously described3.

    Recombinant:

    Article Title: MPA PASS software enables stitched multiplex, multidimensional EV repertoire analysis and a standard framework for reporting bead-based assays
    Article Snippet: CD10 , 0.5 μg , APC , mouse IgG1κ , 97C5 , Miltenyi Biotec , 130-093-450. .. CA9 , 0.5 μg , APC , recombinant human IgG1 , REA658 , Miltenyi Biotec , 130-110-058. .. CD314 , 0.5 μg , APC , mouse IgG1κ , 1D11 , BioLegend , 320808.

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    Image Search Results


    GA inhibits TNBC growth via targeting CA9. (a) Venn diagram showing the number of differentially expressed genes in the comparison between MDA-MB-231 and BT549. (b,c) TCGA and GEO databases show that CA9 is highly expressed in TNBC. (d) Correlation analysis between TNBC patient prognosis and CA9 expression. (e) IHC staining of TNBC patient-derived cancer tissue and adjacent tissue. Scale bar: 100 μm. (f) The quantitative analysis of CA9. (g) The protein levels of CA9 in cell lines were measured by Western blotting. (h) The predicted binding mode of GA with CA9. (i) GA binds to CA9 protein as shown by SPR. (j,k) CETSA analysis of CA9 combined with GA. (l,m) CA9 mRNA levels after GA 24 h treatment by qRT-PCR. (n,o) CA9 protein levels after GA 24 h treatment by Western blotting. (p) The related protein levels of siRNA-CA9 TNBC cells after GA 24 h treatment by Western blotting. Data are presented as mean ± SD. n = 3. * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001 when compared with control group.

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: Garciniagifolone A, derived from Garcinia multiflora fruits, inhibits triple-negative breast cancer cells and organoids growth by targeting CA9

    doi: 10.3389/fcell.2026.1767397

    Figure Lengend Snippet: GA inhibits TNBC growth via targeting CA9. (a) Venn diagram showing the number of differentially expressed genes in the comparison between MDA-MB-231 and BT549. (b,c) TCGA and GEO databases show that CA9 is highly expressed in TNBC. (d) Correlation analysis between TNBC patient prognosis and CA9 expression. (e) IHC staining of TNBC patient-derived cancer tissue and adjacent tissue. Scale bar: 100 μm. (f) The quantitative analysis of CA9. (g) The protein levels of CA9 in cell lines were measured by Western blotting. (h) The predicted binding mode of GA with CA9. (i) GA binds to CA9 protein as shown by SPR. (j,k) CETSA analysis of CA9 combined with GA. (l,m) CA9 mRNA levels after GA 24 h treatment by qRT-PCR. (n,o) CA9 protein levels after GA 24 h treatment by Western blotting. (p) The related protein levels of siRNA-CA9 TNBC cells after GA 24 h treatment by Western blotting. Data are presented as mean ± SD. n = 3. * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001 when compared with control group.

    Article Snippet: The CA9 protein (Cusabio, CSB-EP614990HUc0) was coupled to the surface of CM5 chip biosensor (Cytiva, 29104988) in 10 mM sodium acetate (pH 4.5).

    Techniques: Comparison, Expressing, Immunohistochemistry, Derivative Assay, Western Blot, Binding Assay, Quantitative RT-PCR, Control

    Model of the anti-tumor mechanism of GA in TNBC cells. GA could bind CA9 to induce early stage autophagy by blocking PI3K/AKT/mTOR signaling pathway. Moreover, GA suppressed the autophagic flux and increased the p62 to promote ROS production, which activated NLRP3/caspase-1/GSDMD/caspase-3 related pyroptosis and apoptosis to induce TNBC cells death.

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: Garciniagifolone A, derived from Garcinia multiflora fruits, inhibits triple-negative breast cancer cells and organoids growth by targeting CA9

    doi: 10.3389/fcell.2026.1767397

    Figure Lengend Snippet: Model of the anti-tumor mechanism of GA in TNBC cells. GA could bind CA9 to induce early stage autophagy by blocking PI3K/AKT/mTOR signaling pathway. Moreover, GA suppressed the autophagic flux and increased the p62 to promote ROS production, which activated NLRP3/caspase-1/GSDMD/caspase-3 related pyroptosis and apoptosis to induce TNBC cells death.

    Article Snippet: The CA9 protein (Cusabio, CSB-EP614990HUc0) was coupled to the surface of CM5 chip biosensor (Cytiva, 29104988) in 10 mM sodium acetate (pH 4.5).

    Techniques: Blocking Assay